Pseudotype viruses - applications and troubleshooting
نویسنده
چکیده
Pseudotype viruses are rapidly establishing themselves as important research and diagnostic tools of basic and clinical scientists facilitating the detailed study of individual viral genes, host cell receptors and highly pathogenic viruses, circumventing the need for high-level biosafety containment. The switching of surface envelope proteins expressed on the surface of these pseudotypes enables them to be used as surrogate viruses in neutralization/antiviral screening assays and for the study of cell–virus receptor interactions. This meeting encompasses the many diverse applications of pseudotype technologies from a practical, translational and public health perspective. This event has CPD accreditation.
منابع مشابه
Pseudotypes: your flexible friends.
Pseudotype viruses: applications and troubleshooting’ (EuroSciCon 2013), a 1-day conference held at Cineworld: The O2 (London, UK) on 2 October 2013, focused on the technique of pseudotyping enveloped viruses (for a review of the technique, see [1]). The talks and posters covered the challenges and successes of pseudotyping viruses from a broad range of families (Retroviridae, Flaviviridae, Ort...
متن کاملDevelopment and Applications of VSV Vectors Based on Cell Tropism
Viral vectors have been available in various fields such as medical and biological research or gene therapy applications. Targeting vectors pseudotyped with distinct viral envelope proteins that influence cell tropism and transfection efficiency are useful tools not only for examining entry mechanisms or cell tropisms but also for vaccine vector development. Vesicular stomatitis virus (VSV) is ...
متن کاملDifferential diagnosis of feline leukemia virus subgroups using pseudotype viruses expressing green fluorescent protein.
Feline leukemia virus (FeLV) is classified into three receptor interference subgroups, A, B and C. In this study, to differentiate FeLV subgroups, we developed a simple assay system using pseudotype viruses expressing green fluorescent protein (GFP). We prepared gfp pseudotype viruses, named gfp(FeLV-A), gfp(FeLV-B) and gfp(FeLV-C) harboring envelopes of FeLV-A, B and C, respectively. The gfp p...
متن کاملAnalyses of Entry Mechanisms of Novel Emerging Viruses Using Pseudotype VSV System
Emerging infectious diseases include newly identified diseases caused by previously unknown organisms or diseases found in new and expanding geographic areas. Viruses capable of causing clinical disease associated with fever and bleeding are referred to as viral hemorrhagic fevers (VHFs). Arenaviruses and Bunyaviruses, both belonging to families classified as VHFs are considered major etiologie...
متن کاملIsolation of RD-114-like oncornavirus from a cat cell line.
A clone of cells derived from a continuous line of cat cells (CCC) spontaneously produced an RNA C-type virus (CCC virus) which did not have the group-specific antigen of the standard strains of feline leukemia viruses but did have that of the RD-114 virus. Single-hit infection of a virus yielding CCC cell with only the feline leukemia virus pseudotype of murine sarcoma virus [MSV(FeLV)] result...
متن کامل